cd206 polyclonal antibody  (Proteintech)

Journal: Bioactive Materials

Article Title: Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration

doi: 10.1016/j.bioactmat.2025.11.009

Figure Lengend Snippet: Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

Article Snippet: For CD206, cells were stained using a CD206 polyclonal antibody (1:600, 18704-1-AP, Proteintech, USA) and a Cy3-labeled goat anti-mouse IgG (H + L) antibody (1:400, A0521, Beyotime, China), following the same protocol.

Techniques: Staining, Fluorescence, Cell Culture, Marker, Flow Cytometry, Labeling

Journal: Bioactive Materials

Article Title: Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration

doi: 10.1016/j.bioactmat.2025.11.009

Figure Lengend Snippet: In vivo regulation of the immune microenvironment by GM&Zn 2+ /Ce 3+ -WH. A) At postoperative weeks 4 and 8, the immunomodulatory function of scaffolds was assessed using IF staining for a) CD86 and b) CD206 (scale bar = 50 μm). B) Semiquantitative analysis of CD86 + /CD206+ at 4 weeks. C) Semiquantitative analysis of CD86 + /CD206+ at 8 weeks. (n = 6). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

Article Snippet: For CD206, cells were stained using a CD206 polyclonal antibody (1:600, 18704-1-AP, Proteintech, USA) and a Cy3-labeled goat anti-mouse IgG (H + L) antibody (1:400, A0521, Beyotime, China), following the same protocol.

Techniques: In Vivo, Staining

Journal: Materials Today Bio

Article Title: Isoliquiritigenin micellar microneedle for pH monitoring and diabetic wound healing

doi: 10.1016/j.mtbio.2025.102356

Figure Lengend Snippet: Anti-inflammatory mechanism and regulation of polarization of macrophages by LSI-GCA. (a) Immunofluorescence staining for M1 marker CD86 (green: CD86, blue: DAPI). Scale bar: 100 μm. (b) Quantitative statistics of CD86. (c) Immunofluorescence staining of M2 marker CD206 (green: CD206, blue: DAPI). Scale bar: 100 μm. (d) Quantitative statistics of CD206. (e) NRF2 nuclear translocation assay (red: NRF2, blue: DAPI). Scale bar: 100 μm. (f) Quantitative statistics of NRF2 nuclear translocation assay. Data are presented as mean ± standard deviation (n = 3). (Statistical differences: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, NS, no statistical significance). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Human umbilical vein endothelial cells (HUVEC), mouse fibroblasts (3T3), and mouse macrophage RAW264.7 cells were supplied by Wuhan Pulisai Biotechnology Co., Ltd. Polyclonal antibodies against CD206 and CD86, plus CoraLite488-conjugated goat anti-rabbit IgG(H + L), were bought from Proteintech.

Techniques: Immunofluorescence, Staining, Marker, Nuclear Translocation Assay, Standard Deviation

Journal: Materials Today Bio

Article Title: Isoliquiritigenin micellar microneedle for pH monitoring and diabetic wound healing

doi: 10.1016/j.mtbio.2025.102356

Figure Lengend Snippet: LSI-GCA enhances wound angiogenesis and downregulates inflammatory factor expression. (a) TNF-α expression in skin wound tissues. Scale bar: 100 μm. (b) IL-6 expression in skin wound tissues. Scale bar: 100 μm. (c) Immunofluorescence staining of CD31 and α-SMA. Scale bar: 100 μm. (d) Immunofluorescence staining of CD206 and F4/80. Scale bar: 100 μm. (e) Quantitative analysis of TNF-α expression. (f) Quantitative analysis of IL-6 expression. (g) Quantitative analysis of CD31 expression. (h) Quantitative analysis of CD206 expression. Data are expressed as mean ± standard deviation (n = 3). (Statistical significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001; NS, not significant).

Article Snippet: Human umbilical vein endothelial cells (HUVEC), mouse fibroblasts (3T3), and mouse macrophage RAW264.7 cells were supplied by Wuhan Pulisai Biotechnology Co., Ltd. Polyclonal antibodies against CD206 and CD86, plus CoraLite488-conjugated goat anti-rabbit IgG(H + L), were bought from Proteintech.

Techniques: Expressing, Immunofluorescence, Staining, Standard Deviation

Journal: Bioactive Materials

Article Title: Bifunctional adECM bioscaffold with STIM1-ASCs and IGF-2 promotes functional masseter VML repair via myogenesis and fibrosis suppression

doi: 10.1016/j.bioactmat.2025.08.019

Figure Lengend Snippet: IGF-2-loaded adECM scaffolds regulate macrophage polarization and metabolic reprogramming. (A) Schematic diagram of the experimental setup using a transwell co-culture system to study macrophage polarization induced by IGF-2-loaded adECM scaffolds. (B) IGF-2 release curves from adECM scaffolds loaded with different concentrations of IGF-2 (5 ng/mL, 10 ng/mL, and 20 ng/mL), n = 3. (C) Flow cytometric analysis of macrophages showing the expression of CD206 + (M2 marker) gated cells after 72 h of different treatments. (D) Corresponding statistical analysis of flow cytometry data. Data are presented as mean ± s.d. (n = 3). Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group. (E and F) RT-qPCR analysis of M2 related gene (IL-10 and Arg-1) expression levels after different treatments on day 3. (G) The extracellular acidification rate (ECAR) was measured using the Seahorse XF96 Extracellular Flux Analyzer, and statistical results for Glycolysis, Glycolytic capacity and Glycolytic reserve are shown in (H). Abbreviations: 2-DG, 2-deoxy-D-glucose; Rot/AA, rotenone/antimycin A. n = 5 per group. (I) The oxygen consumption rate (OCR) and statistical results for basal respiration, maximal respiration, and spare respiratory capacity, detected by the Seahorse XF96 Analyzer. (J) Statistical results for respiration parameters. Abbreviations: FCCP, carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone; Rot/AA, rotenone/antimycin A. n = 5 per group. All data are presented as mean ± s.d. Statistical significance was determined using one-way ANOVA: ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, compared with the Control group.

Article Snippet: After 72 h of co-culture, the collected cells were incubated with CD80-PE antibody (1:200, 12-0800-82, eBioscience), CD206-APC (MRC1/APC) antibody (1:100, bs-4727R, Bioss), and F4/80-FITC antibody (1:200, AER-051-F, ThermoFisher) for 30 min.

Techniques: Co-Culture Assay, Expressing, Marker, Flow Cytometry, Control, Quantitative RT-PCR